COST EFFECTIVE PROCESS FOR THE PRODUCTION OF FUNGAL L-ASPARAGINASES FROM PENCILLIUM SPS. ISOLATED FROM LOCAL SOIL SAMPLE
L â€“ asparaginase is known to have a therapeutic function and was used for the patients alleged from acute lymphoblastic leukemia (ALL). Out of different micro organisms, fungal samples isolated from a local soil sample, Pencillium sps. 02 have utmost L-asparaginase activity. In the present study, Pencillium sp. 02 was isolated and studied for enhanced enzyme production with the help of statistical methods. Initially, all nutritional factors were selected by one factor- at-a-time method (171 IU/ml), then the consequence of every factor with respect to production of L-asparaginase was identified by taguchi design and the enzyme yield was increased to 1.073 folds (171 IU/ml to 183.5 IU/ml). Later, final optimization was carried out with response surface methodology (Central Composite Design) increased the yield of 1.065 folds (183.5 IU/ml to 195.5 IU/ml).
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