COST EFFECTIVE PROCESS FOR THE PRODUCTION OF FUNGAL L-ASPARAGINASES FROM PENCILLIUM SPS. ISOLATED FROM LOCAL SOIL SAMPLE

  • SR Kotra Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
  • N Prudvi Department of Biotechnology, R.V.R & J.C College of Engineering, Chowdavaram, Guntur, Andhra Pradesh, India
  • Kra Sada Sai Department of Biotechnology, R.V.R & J.C College of Engineering, Chowdavaram, Guntur, Andhra Pradesh, India
  • JB Peravali Department of Biotechnology, Bapatla College of Engineering, Bapatla, Andhra Pradesh, India.
  • Anmol Kumar Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
  • Krs Sambasiva Rao Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
  • K K Pulicherla Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India

Abstract

L – asparaginase is known to have a therapeutic function and was used for the patients alleged from acute lymphoblastic leukemia (ALL). Out of different micro organisms, fungal samples isolated from a local soil sample, Pencillium sps. 02 have utmost L-asparaginase activity. In the present study, Pencillium sp. 02 was isolated and studied for enhanced enzyme production with the help of statistical methods. Initially, all nutritional factors were selected by one factor- at-a-time method (171 IU/ml), then the consequence of every factor with respect to production of L-asparaginase was identified by taguchi design and the enzyme yield was increased to 1.073 folds (171 IU/ml to 183.5 IU/ml). Later, final optimization was carried out with response surface methodology (Central Composite Design) increased the yield of 1.065 folds (183.5 IU/ml to 195.5 IU/ml).

Author Biographies

SR Kotra, Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
N Prudvi, Department of Biotechnology, R.V.R & J.C College of Engineering, Chowdavaram, Guntur, Andhra Pradesh, India
Department of Biotechnology, R.V.R & J.C College of Engineering, Chowdavaram, Guntur, Andhra Pradesh, India
Kra Sada Sai, Department of Biotechnology, R.V.R & J.C College of Engineering, Chowdavaram, Guntur, Andhra Pradesh, India
Department of Biotechnology, R.V.R & J.C College of Engineering, Chowdavaram, Guntur, Andhra Pradesh, India
JB Peravali, Department of Biotechnology, Bapatla College of Engineering, Bapatla, Andhra Pradesh, India.
Department of Biotechnology, Bapatla College of Engineering, Bapatla, Andhra Pradesh, India.
Anmol Kumar, Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
Krs Sambasiva Rao, Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
K K Pulicherla, Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India

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Published
2013-04-01
How to Cite
Kotra, S., Prudvi, N., Sai, K. S., Peravali, J., Kumar, A., Rao, K. S., & Pulicherla, K. K. (2013). COST EFFECTIVE PROCESS FOR THE PRODUCTION OF FUNGAL L-ASPARAGINASES FROM PENCILLIUM SPS. ISOLATED FROM LOCAL SOIL SAMPLE. Mintage Journal of Pharmaceutical and Medical Sciences (ISSN: 2320-3315), 45-50. Retrieved from http://mjpms.in/index.php/mjpms/article/view/119