• Alouani abdelouaheb abdelouaheb UNIV SOUK AHRAS
  • Rehimi Nassima Department of Biological Sciences, University of Badji Mokhtar Annaba -23000, Algeria.
  • Soltani Noureddine Department of Biological Sciences, University of Badji Mokhtar Annaba -23000, Algeria.


 Introduction : The mosquito Culex pipiens L is a common and abundant species in the world. This species serve as bridge vectors of the West Nile virus from birds to humans. Objective: Under the laboratory conditions, toxicological evaluation of two compounds Azadirachtin and Bacillus thuringiensis israelensis ( B.t.i ) against eggs of  mosquito Cx. pipiens  and biological effect of these compounds on larvae, pupae and adult emergence Methods :  The ovicidal activity was determined after 24 h of exposure to  concentrations of Azadirachtin and B.t.i. Results: indicated high reduction in percentage of egg hatch resulted from treated eggs by azadirachtin, estimated by 31.41% at 1mg /L and 91.51% at 0.125 mg /L, the obtained results show a high mortality with the increase in the concentration,  and prolongation in larval and pupal developments resulted from treated eggs by azadirachtin, estimated by 4.55 days at 0.125mg/L and 7.66 days at 1 mg/L in first instar , larval and pupal duration increased with the increase in the concentration of azadirachtin. The results indicated high augmentation in the percentages of the malformations with the decrease in the concentration of azadirachtin. In contrast, in adult stage, the results indicated high reduction in total eggs laid, percentage of hatchability and longevity. No effect was observed after treated eggs by B.t.i.  Conclusion:From the results, it can   be concluded the Azadirachtin are excellent potential for controlling the mosquito Culex pipiens.

Author Biographies

Alouani abdelouaheb abdelouaheb, UNIV SOUK AHRAS
Rehimi Nassima, Department of Biological Sciences, University of Badji Mokhtar Annaba -23000, Algeria.
Department of Biological Sciences, University of Badji Mokhtar Annaba -23000, Algeria.
Soltani Noureddine, Department of Biological Sciences, University of Badji Mokhtar Annaba -23000, Algeria.
Department of Biological Sciences, University of Badji Mokhtar Annaba -23000, Algeria.


Akiner MM, Simsek FM, Caglar SS (2009) Insecticide resistance of Culex pipiens (Diptera: Culicidae) in Turkey. J Pestic Sci 34 : 259-264.

Amer AE A (2004) Ecological and physiological studies on bollworms. Ph.D. Thesis, Fac. of Agric. Moshtohor, Benha Univ.

Armengol G, Hernandez J, Velez JG, Orduz S (2006) Long-lasting effects of a Bacillus thuringiensis serovar israelensis experimental tablet formulation for Aedes aegypti (Diptera: Culicidae) control. J Econ Entomol 99(5): 1590-1595.

Baskar K, Ignacimuthu S (2012a) Ovicidal activity of Atalantia monophylla (L) Correa against Helicoverpa armigera Hubner (Lepidoptera: Noctuidae). J Agric Technol 8: 861-868.

Cetin H, Yanikoglu A (2004) Mosquito (Diptera: Culicidae) species, their breeding sites and some biological aspects of dominant species Culex pipiens, Linnaeus in Antalya. Turkish J Entomol Res Soc 28: 283-294.

Cetin H, Yanikoglu A, Cilek JE (2005) Evaluation of the naturally derived insecticide spinosad, against Culex pipiens L. (Diptera: Culicidae) larvae in septic tank water in Antalya, Turkey. J Vector Ecol 30: 151-154.

De-Ling M, Yoshito S, Hiroaki T, Tomonari W, Mami I (2005) Ovicidal and ovipositional effects of neem (Azadirachta indica A. Juss.) extracts on rice bug, Leptocorisa chinensis (Dallas). International Journal of Pest Management 51(4):265-271.

Dhiman RC, Pahwa S, Dhillon GP, Dash AP (2010) Climate change and threat of vector-borne diseases in India: are we prepared. Parasitol Res 106(4): 763-773.

Geetha I, Manonmani AM (2010) A novel mosquitocidal biosurfactant produced by Bacillus suB.t.ilis sp. suB.t.ilis (VCRCB471) and influence of abiotic factors on its pupicidal efficacy. Let Appl Microbial 51(4): 406-412.

Govindarajan M (2010) Evaluation of indigenous plant extracts against the malarial vector, Anopheles stephensi (Liston) (Diptera: Culicidae). Parasitol Res doi:10.1007/s00436-010-2224-0.

Gunasekaran K, Boopathi Doss PS, Vaidyanathan K (2004) Laboratory and field evaluation of Teknar HP-D, a biolarvicidal formulation of Bacillus thuringiensis ssp. israelensis, against mosquito vectors. Acta Trop 92: 109–118.

Hamer GL, Kitron UD, Brawn JD, Loss SR, Ruiz MO, Goldberg TL(2008)Culex pipiens (Diptera: Culicidae): a bridge vector of West Nile virus to humans. J Med Entomol 45(1):125-128.

Hewady AA, Kandel M. A, El-Sayed A A , Rashad A M (2002) Biological and Biochemical effects of Neemazal T/S on Pink bollworm Pectinophora gossypiella ( Saunders) (Lepidoptera: Gelechiidae). J. Egypt. Acad. Soc. Environ. Develop 2(2): 53-65.

Likitvivatanavong S, Chen J, Bravo A, Soberon M, Gill SS (2011) Cadherin, alkaline phosphatase, and aminopeptidase N asreceptors of Cry11Ba toxin from Bacillus thuringiensis subsp. in Aedes aegypti. Appl Environ Microbiol 77(1): 24-31.

Lyra SRM, Ferraz JMG , Silva APP(1999) Action of chitin synthesis inhibitor on reproduction of Spodoptera littoralis. Review of Agric. Entomol 87-95.

Maheswaran R, Ignacimuthu S (2012) A novel herbal formulation against dengue vector mosquitoes Aedes aegypti and Aedes albopictus. Parasitol Res 110:1801-1813.

Moawad GM, Khidr AA (1982) The influence of juvenil hormone the pink bollworm, Pectinophora gossypiella (Saund.). Agric. Res. Rev 60:225-235.

Nathan SS, Kalaivani K, Murugan K (2005) Effects of neem limonoids on the malaria vector Anopheles stephensi Liston (Diptera: Culicidae). Acta Trop 96(1): 47-55.

Nathan SS, Kalaivani K, Murugan K (2006) Behavioural responses and changes in biology of rice leaf-folder following treatment with a combination of bacterial toxins and botanical insecticides. Chemosphere 64(10): 1650-1658.

Packiam SM, Ignacimuthu S (2012) Effect of PONNEEM on Spodoptera litura (Fab.) and Its Compatibility with Trichogramma chilonis Ishii. Braz Arch Biol Technol 22:291-298.

Poncet S, Delecluse A, Klier A, Rapoport G (1995) Evaluation of synergistic interactions among the CryIVa, CryIVb and CryIVd toxic components of Bacillus thuringiensis subsp. Israelensis crystals. J Inverte Pathol 66(2): 131-135.

Sammour EA, Kandil MA, Abdel-Aziz, NF (2008) The reproductive potential and Fate of chlorfluazuron and leufenuon against cotton leafworm, Spodoptera littoralis (Boisd). American- Eurasian J. Agric Environ Sci 4 (1) 62-67.

Senthil NS (2005) Effects of Melia azadirach on nutritional physiology and enzyme activitie of the rice leaf folder Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Pyralidae). Pest. Biochem and Physiol.

Shaalan EA, Canyon D, Younes MW, Abdel-Wahab H, Mansour AH(2005).A review of botanical phytochemicals with mosquitocidal potential. Environ International 31(8):1149-66.

Soliman BA, Tewfieck MA (1999) Activity and efficacy of Azadirachtin (Neem product) on the eggs of the filarial vector, Culex pipiens (Diptera: Culicidae). Journal of the Union of Arab Biologist Cairo 12 (A): 33-41.

Su T, Mulla MS (1999) Oviposition bioassay response of Culex tarsalis and Culex quenquifasciatus to neem products containing azadirachtin. Entomologia Experimentalis Applica. 82: 337-340

Wirth MC, Park HW, Walton WE, Federici BA (2005) Cyt1A of Bacillus thuringiensis delays evolution of resistance to Cry11A in the mosquito Culex quinquefasciatus. Appl Environ Microbiol 71(1): 185-189.

Yin XH, Wu QJ, Li XF, Zhang YJ, Xu BY (2008) Sublethal effects of spinosad on Plutella xylostella (Lepidoptera: Yponomeutidae). Crop Protection 27:1385-1391.

Zalizniak L, Nugegod D (2006) Effect of sublethal concentrations of chlorpyrifos on three successive generations of Daphnia carinata. Ecotoxicol. Environ. Saf 64: 207-214.

Zebtiz CPW (1987) Potential of neem seed kernel extracts in mosquito control. Proceedings of third international Neem conference

How to Cite
abdelouaheb, A. abdelouaheb, Nassima, R., & Noureddine, S. (2018). OVICIDAL ACTIVITY OF AZADIRACHTIN AND BACILLUS THURINGIENSIS ISRAELENSIS AGAINST EGGS OF MOSQUITO CULEX PIPIENS. Mintage Journal of Pharmaceutical and Medical Sciences (ISSN: 2320-3315), 8-12. Retrieved from http://mjpms.in/index.php/mjpms/article/view/347