• Emmanuel Oluwarotimi Adekunle Ajasin University, Akungba Akoko
Keywords: D. melanogaster, T. tetraptera, Malondialdehyde, Superoxide dismutase, Catalase


Tetrapleura tetraptera (TT) known as Aridan in Yoruba (Nigeria) has been used in folklore for ages. This study examined the phytochemical composition of TT and antioxidant effects of its dietary inclusion in Drosophilia melanogaster. Experiments with D.melanogaster as an animal model were conducted. Dried TT fruit was ground into powder. The antioxidant effects of dietary inclusion of TT on D. melanogaster and mRNA expression of antioxidant genes by real time-PCR assay were also determined. Phytochemical screening showed the presence of important secondary metabolites. The results indicate that TT fruit exhibited antioxidant property by reducing production of Malondialdehyde (MDA) and up-regulating superoxide dismutase (SOD) and  catalase (CAT) expression significantly (p < 0.05) in D. melanogaster groups fed with 0.1% and 1% concentrations of TT compared to the negative control. These findings further established the antioxidant protective potential of TT in folklore.



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