PHYTOCHEMICAL ANALYSIS OF TETRAPLEURA TETRAPTERA FRUIT AND ANTIOXIDANT ACTIVITIES OF ITS DIETARY INCLUSION IN DROSOPHILA MELANOGASTER

  • Emmanuel Oluwarotimi Adekunle Ajasin University, Akungba Akoko
Keywords: D. melanogaster, T. tetraptera, Malondialdehyde, Superoxide dismutase, Catalase

Abstract

Tetrapleura tetraptera (TT) known as Aridan in Yoruba (Nigeria) has been used in folklore for ages. This study examined the phytochemical composition of TT and antioxidant effects of its dietary inclusion in Drosophilia melanogaster. Experiments with D.melanogaster as an animal model were conducted. Dried TT fruit was ground into powder. The antioxidant effects of dietary inclusion of TT on D. melanogaster and mRNA expression of antioxidant genes by real time-PCR assay were also determined. Phytochemical screening showed the presence of important secondary metabolites. The results indicate that TT fruit exhibited antioxidant property by reducing production of Malondialdehyde (MDA) and up-regulating superoxide dismutase (SOD) and  catalase (CAT) expression significantly (p < 0.05) in D. melanogaster groups fed with 0.1% and 1% concentrations of TT compared to the negative control. These findings further established the antioxidant protective potential of TT in folklore.

 

References

1. Abolaji OA, Kamdem JP, Lugokenski TH, Nascimento TK, Waczuk, EP, Farombi EO and Rocha JBT. Involvement of oxidative stress in 4-vinylcyclohexene- induced toxicity in Drosophila melanogaster. Free Radical Biology and Medicine, 2014; 71, 99–108. https:// doi.org/10.1016/j.freeradbiomed.2014.03.014
2. Adedara IA, Klimaczewski CV, Barbosa NB, Farombi EO, Souza DO and Rocha JBT. Influence of diphenyl diselenide on chlorpyrifos-induced toxicity in Drosophila melanogaster. Journal of Trace Elements in Medicine and Biology, 2015a; 32, 52–59. https://doi. org/10.1016/j.jtemb.2015.05.003
3. Adedara IA, Rosemberg DB, Souza DO, Kamdem JP, Farombi EO, Aschner M and Rocha JBT. Biochemical and behavioral deficits in lobster cockroach Nauphoeta cinerea model of methylmercury exposure. Toxicology Research, 2015b; 4, 442–451. https:// doi.org/10.1039/C4TX00231H
4. Baker KD and Thummel CS. Diabetic larvae and obese fliese merging studies of metabolism in Drosophila. Cell Metabolism, 2007; 6, 257–266. https://doi.org/10.1016/j.cmet.2007.09.002
5. Benford DJ, Hanley AB, Bottrill K, Oehlschlager S, Balls M, Brance F and Schilter B. Biomarkers as predictive tools in toxicity testing. The report and recommendations of ECVAM workshop 40. Alternatives to Laboratory Animals, 2000; 28, 119–131
6. Fusco D, Colloca G, Lo Monaco MR and Cesari M. Effects of antioxidant supplementation on the aging process. Clin. Interv. Aging. 2007; 2: 377- 387.
7. Halliwell B. Oxidative stress and neurodegeneration: where are we now? J Neurochem. 2006; 97:1634–1658.
8. Sharma P, Jha AB, Dubey RS and Pessarakli M. Reactive oxygen species, oxidative damage and antioxidative defense mechanism in plants under stressful conditions. J. Bot. 2012; 10.1155/2012/217037.
9. Pham-Huy LA, He H and Pham-Huy C. Free radicals, antioxidants in disease and health. Int. J. Biomed. Sci. 2008; 4: 89-96.
10. Wiernsperger NF. Oxidative stress as a therapeutic target in diabetes: revisiting the controversy. Diabetes Metab. 2003; 29: 579–585.
11. Eboh A. “Biochemistry of free radicals and antioxidants,” Scholars Academic Journal of Biosciences, 2014; vol. 2, pp. 110–118.
12. Pauline N, Cabral BNP, Anatole PC, Jocelyne AMV, Bruno M and Jeanne NY. “The in vitro antisickling and antioxidant effects of aqueous extracts Zanthoxyllum heitzii on sickle cell disorder,” BMC Complementary and Alternative Medicine 2013; vol. 13, article 162.
13. Rahman K. Studies on free radicals, antioxidants and co-factors. Clin. Inverv. Aging 2007; 2: 219–236.
14. Wachtel-Galor S and Benzie IFF. Herbal Medicine: An Introduction to its History, Usage, Regulation, Current Trends and Research Needs. In: Herbal Medicine: Biomolecular and Clinical Aspects, Benzie IFF, Wachtel-Galor S. (Eds.). 2nd Edn. CRC Press, Boca Raton, FL, USA; 2011. Chapter 1, p. 1-10
15. Orwa C, Mutua A, Kindt R, Jamnadass R and Simons A. Agroforestree database. A tree reference and selection guide, version 4.0. World Agroforestry Centre, Kenya. 2009
16. Pandey N, Meena RP, Rai SK and Pandey-Rai S. “Medicinal plants derived nutraceuticals: a re-emerging health aid,” International Journal of Pharma and Bio Sciences, 2011; vol. 2, pp. 419–441.
17. Irondi AE, Anokam KK and Chukwuma PC. “Phenological variation in the in-vitro antioxidant properties and alpha-amylase inhibitory activity of Tetrapleura tetraptera Pod,” International Journal of Pharmaceutical Sciences and Drug Research, 2013; vol. 5, pp. 108–112.
18. Moukette BM, Pieme AC, Biapa PCN, Njimou JR, Stoller M and Bravi M. In vitro ion chelating, antioxidative mechanism of extracts fromfruits and barks of Tetrapleura tetraptera and their protective effects againstfenton mediated toxicity of metal ions on liver homogenates, Evid.-Based Complement. Altern. Med. 2015; 1–15.
19. Odubanjo VO, Ibukun EO and Oboh G. Toxicological evaluations of aqueous extracts of two Nigerian ethnobotanicals (Tetrapleura tetraptera and Quassia undulata) of neurological importance in rats, Comp. Clin. Pathol. 2017; http://dx.doi.org/10. 1007/s00580-017-2611-3
20. Oboh G, Ogunsuyi OB, Ojelade MT and Akomolafe SF. Effect of dietary inclusions of bitter kola seed on geotactic behavior and oxidative stress markers in Drosophila melanogaster. Food Sci Nutr. 2018; 6:2177-2187.
21. Edeoga HO, Okwu DE and Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr. J. Biotechnol. 2005; Vol. 4 (7): 685-688.
22. Ohkawa H, Ohishi N and Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95:351–358.
23. Harb A, Award D and Samarah N. Gene expression and activity of antioxidant enzymes in barlry (Hordeum vulgare L.) under controlled severe drought. Journal of Plant Interactions, 2015; 10:109-116.
24. Zar JH. Biostatistical Analysis. Prentice-Hall, Inc., Upper Saddle River, NJ 1984; p. 620.
25. Xi M, Hai C, Tang H, Chen M, Fang K and Liang X. Antioxidant and antiglycation properties of total saponins extracted from traditional chinese medicine used to treat diabetes mellitus, Phytother. Res. 2008; 22: 228–237.
26. Menkem EZ, Fokou JH, Tsague IF and Chouadeu PM. “Antifungal and antioxidant activities of Piptostigma calophyllum, Uvariodendron calophyllum and Uvariodendron molundense growing in Cameroon,” Journal of Biologically Active Products from Nature, 2012; vol. 2, no. 2, pp. 110–118.
27. Oboh G, Akinyemi AJ and Ademiluyi SO. Antioxidant properties and inhibitory effect of ethanolic extracts of Struchium sparganophora (Ewuro Odo) Leaf on α – Amylase and α – Glucosidase Activities. Afr J Tradt Complement Altern Med. 2012; 9(3):342-39.
28. Bruno MM, Anatole PC, Cabral BN, Romain NJ and Yonkeu NJ. “Free radicals quenching potential, protective properties against oxidative mediated ion toxicity and HPLC phenolic profile of a Cameroonian spice: Piper guinneensis,” Toxicology Reports, 2015.
29. Ighodaro OM and Akinloye OA. First line defense antioxidants superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defense grid. Alexandria Journal of Medicine. In press (2017)
30. Shanthi S, Parasakthy K, Deepalakshmi PD and Devaraj SN. Hypolipidemic activity of tincture of Crataegus in rats. Indian J Biochem Biophys, 1994; 31: 143–146.
Published
2020-05-14
How to Cite
Oluwarotimi, E. (2020). PHYTOCHEMICAL ANALYSIS OF TETRAPLEURA TETRAPTERA FRUIT AND ANTIOXIDANT ACTIVITIES OF ITS DIETARY INCLUSION IN DROSOPHILA MELANOGASTER. Mintage Journal of Pharmaceutical and Medical Sciences (ISSN: 2320-3315). Retrieved from http://mjpms.in/index.php/mjpms/article/view/513
Section
Original Article(s)