A COMPARATIVE EVALUATION OF PHYTOCHEMICAL AND ANTIBACTERIAL PROPERTIES OF RICINUS COMMUNIS LINN AND THEVETIA PERUVIANA SCHUM. OF KUMAUN HIMALAYA

Parikshit Kumar, S Joshi, S C Sati, D Rai

Abstract


Objective: The aim of the present study was to evaluate the Ricinus communis and Thevetia peruviana for their phytochemical constituents and antibacterial activity. Methods: Successive hexane, chloroform, ethanol, methanol and aqueous plant extracts of these plants were screened for phytochemical constituents and antibacterial potential. R. communis bark and T. peruviana leaves were tested against a panel of microorganisms (Bacillus subtilis, Escherichia coli, Agrobacterium tumefaciens, Xanthomonas phaseoli and Erwinia chrysanthemi) by the disc-diffusion method. Results: Phytochemical analysis of R. communis extracts revealed the presence of saponins, phenols, flavonoids, quinines, and steroids. On the other hand, T. peruviana indicates that all extracts contained terpenoids, phenols, flavonoids, anthraquinones and free amino acids. The studied extracts of these plants displayed various degrees of antibacterial activities. The extracts of T. peruviana leaves showed the best spectra of activity against all tested microorganisms. The methanol extract was found to be most effective showing the largest zone of inhibition against A. tumefaciens (30±0.6 mm) and E. coli (28±0.3 mm). While methanolic extract of R. communis bark showed its highest inhibitory activity against B. subtilis (18±1.6 mm). The hexane extract of R. communis showed minimum antibacterial activity compared to all other extracts. Conclusion: The results of this study scientifically validate the inhibitory capacity of the above said medicinal plants attributed to their common use in folk medicine which might be useful in the development of new drugs based on natural resources.  


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References


Das S, Pal S, Mujib A and Dey S. “Biotechnology of Medicinal Plants-Recent Advances and Potential,” first ed. vol. 2. UK992 Publications, Hyderabad, 1999. p. 126–139.

Westh H, Zinn CS and Rosdahl VT. Sarisa study Group. An International multicenter study of antimicrobial consumption and resistance in Staphylococcus aureus isolates from 15 hospitals in 14 countries. Micro Drug Resis, 2004; 10: 169-176.

Avila MN, Said D and Ojcius M. “The book reopened on infectious diseases,” Microbes Infection 2008; 10 (9): 942–947.

Kumar VP, Chauhan NS, Padh H and Rajani M. “Search for antibacterial and antifungal agents from selected Indian medicinal plants,” J of Ethnopharm. 2006; 107: 182–188.

Sati SC and Joshi S. “Aspects of the antifungal potential of ethnobotanically known medicinal plants,” Res J Med Plant, 2011; 5 (4): 377-391.

Ahmad I and Beg AZ. “Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens,” J Ethnopharm. 2001; 74: 113-123.

Ukaegbu-Obi KM, Meribe MO and Odo CE. Assessment of antimicrobial activity of aqueous and ethanolic extracts of Monodora myristica (Ehuru) seeds. Mint. J Pharmaceut. & Medical Sci. 2015; 5 (3): 1-3.

Taylor PW. “Alternative natural sources for a new generation of antibacterial agents,” Int J Anti Age, 2013; 42: 195–201.

Sati SC and Kumar P. “Assessment of Himalayan juniper, Juniperus squamata buch– ham ex d. don for phytochemical screening and antimicrobial potential against some infection causing pathogens,” W J Pharma Res. 2015; 4 (10): 998-1011.

Sati SC, Takuli P, Kumar P and Khulbe K. “Antibacterial activity of three medicinal plants of Kumaun Himalaya against some pathogenic bacteria,” Int J Phar Sci and Res, 2015; 6 (11): 1361-1368.

Raoof MA and Yasmeen M. “Aetiology, epidemiology and management of Botrytis grey mold of Castor, Ricinus communis L.,” A review. J Oilseeds Res, 2006; 23 (2): 144-150.

Wang HX and Ng TB. “Examination of lectins, polysaccharopeptide, polysaccharide, alkaloid, coumarin and trypsin inhibitors for inhibitory activity against human immunodeficiency virus reverse transcriptase and glycohydrolases,” Planta Medica, 2001; 67 (7): 669–672.

Isichei CO, Das SC and Ogunkeye OO. “Preliminary clinical investigation of the contraceptive efficacy and chemical pathological effects of RICOM-1013-J of Ricinus communis var minor on women volunteers,” Phytotherapy Res, 2000; 14 (1): 40–42.

F. K. Okwuasaba, U. A. Osunkwo, and M. M. Ekwenchi, “Anticonceptive and estrogenic effects of a seed extract of Ricinus communis var. minor,” J Ethnopharm. 1991; 34 (2-3): 141–145.

Sandhyakumary K, Bobby RG and Indira M. “Antifertility effects of Ricinus communis (Linn) on rats,” Phytotherapy Res, 2003; 17 (5): 508–511.

Ferraz AC, Angelucci MEM, Da Costa ML, Batista IR, De Oliveira BH and Da Cunha C. Pharmacological evaluation of ricinine, a central Nervous system stimulant isolated from Ricinus communis, Pharm Biochem Behav, 1999; 63 (3): 367–375.

Upasani SM, Kotkar HM, Mendki PS and Maheshwari VL. “Partial characterization and insecticidal properties of Ricinus communis L. foliage flavonoids,” Pest Manag Sci, 2003; 59 (12): 1349–1354.

Khan JA and Yadav KP. “Assessment of antifungal properties of Ricinus communis,” J Pharm and Biomed Sci, 2011; 11 (2): 1-3.

Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN and Ray C. “Screening of Indian plants for biological activity. Part I,” In J Exper Bio. 1968; 6: 232-247.

Ali M, Ravindra E and Ramachandram R. “New ursane-type triterpenic esters from the stem bark of Thevetia peruviana,” Pharmazie, 2000; 55 (5): 385-389.

Tewtrakul S, Nakamura N, Hattori M, Fujiwara T and Supavita T. “Flavanone and Flavonol Glycosides from the Leaves of Thevetia peruviana and Their HIV-1 Reverse Transcriptase and HIV-1 Integrase Inhibitory Activities,” Chem. Pharm. Bull. 2002; 50 (5): 630-635.

Kareru PG, Keriko JM, Kenji GM and Gachanja AN. “Anti-termite and antimicrobial properties of paint made from Thevetia peruviana (Pers.) Schum. oil extract,” Afr J Phama and Pharmaco. 2010; 4 (2): 87-89.

Panigrahi A and Raut SK. “Thevetia peruviana (Family: Apocynaceae) in the control of slug and snail pests,” Mem. Inst. Oswaldo Cruz, Rio de Janeiro. 1994; 89 (2): 247-250.

McLaughlin JL, Freedman B, Powel RG and Smith CR. “Neriifolin and 2’acetylneriifolin. Insecticidal and cytotoxic agents of Thevetia theveotides seeds,” J. Econ. Entomol. 1980; 73: 398-402.

Langford SD and Boor PJ. “Oleander Toxicity: An examination of human and animal toxic exposures,” Toxicology, (Medline), 1966; 109: 1-13.

Mendoza JDV, Pumarola T, Gonzales LA and Del Valle LJ. “Antiviral activity of maca (Lepidium meyenii) against human influenza virus,” Asi Paci J Trop Med. 2014; 7 (1): 415-420.

Bauer AW, Kirby WMM, Sherris JC and Turck M. “Antibiotic susceptibility testing by a standardized single disk method,” Am J Clin Path. 1966; 45: 493–496.

Harborne JB. “Phytochemical methods: A guide to modern technique of plant analysis,” Champman and Hall, London, 1998.

Berenbaum MR. “The chemistry of defense: theory and practice. In: Eisner T, Meinwald J, editors. Chemical ecology: the chemistry of biotic interaction,” Washington, DC: National Academy Press, 1995.

Swain T. “Secondary compounds as protective agents,” Ann Rev Plant Physio. 1977; 28: 479–501.

Kaul MK. “High altitude botanicals in integrative medicine- Case studies from Northwest Himalaya,” In J Trad Know. 2010; 9 (1): 18-25.

Sati SC, Kumar P and Joshi S. “The bark extracts of Himalayan gymnosperm Picea smithiana (Wall.): A natural sources of antibacterial and antioxidant agent,” Int J Pharm, Photon, 2015; 106: 445-452.

De Boer HJ, Kool A, Broberg A Mziray WR, Hedberg I and Levenfors JJ. “Antifungal and antibacterial activity of some herbal remedies from Tanzania,” J Ethnopharm. 2005; 96: 461-469.

Sharma M, Mir MI, Malla MY, Mir AH, Nazir S and Tripathi J. “Antimicrobial potential of various extracts of Ricinus communis L.,” Scholars Research Library, , 2013; 3 (2): 72-75.

Joshi S and Sati SC. Antifungal Potential of Gymnosperms: A Review. In “Microbes: Diversity and Biotechnology” (Eds. S. C. Sati and M. Belwal). 2012; p. 333-345.

Khulbe K and Sati SC. Antibacterial activity of Boenninghausenia albiflora Reichb. (Rutaceae). Afr J Biotech. 2009; 8 (22): 6346-6348.

Kensa VM and Syhed Y. “Phytochemical screening and antibacterial activity on Ricinus communis L.,” Plant Sci Feed, 2011; 1 (9): 167-173.

Shannon DL and Paul JB. “Oleander toxicity: an examination of human and animal toxic exposures,” Toxicology, 1996; 109 (1): 1-13.

Sati SC, Khulbe K and Joshi S. “Antibacterial evaluation of the Himalayan medicinal plant Valeriana wallichii DC. (Valerianaceae),” Res J Micro. 2011; 6: 289-296.


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