ACTIVATION OF COMPLEMENT SYSTEM, ALTERNATE PATHWAY ACTIVITY OF SELECTED HERBAL DRUGS

Balaji Pandiyan, Venkatachalam Venkatachalam, Siva Kumar Thillainayagam, Kannan Kamarajan

Abstract


Objective: To screen the selected herb / herbal preparations for their effect on complement system Alternative Pathway activity. Methods: The effect of aqueous extracts of selected eight plants (Aegle marmelos, Withania somnifera, Centella asiatica, Curcuma longa, Cynodon dactylon, Phyllanthus amarus, Solanum trilobatum, Wedelia chinensis  an herbal preparation (Trikattu churanum) and an active constituent (curcumin) on in vitro complement system alternate pathway activity was evaluated and compared with that of known immunostimulant levamisole and negative standard cyclophosphamide. Results: The results suggest that there is a concentration dependent increase in the AP activity for levamisole, AETC, AEST, ACCD, AECA, AEPA, AEWS, AEWC and AEAM. Among the samples investigated for all the eight preparations exhibited increased AP activity though different extent when compared to positive control levamisole. Three preparations namely Cyclophosphamide (CYP-negative control), AECL and AECU did not exhibit any effect on Alternate pathway activity. It may be due to the fact that CYP, AECL and AECU suppress the immune system by different mechanisms and not through alternate pathway. Conclusion: In conclusion, the selected herb / herbal preparations stimulating innate immune system like complement system alternate pathway activity and also it is the better choice for the treatment of infectious disease without causing autoimmune disorder

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